New Publication in Circulation Research

A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. Ackers-Johnson M, Li PY, Holmes AP, O’Brien SM, Pavlovic D, Foo RS. Circulation Research 2016.

See also:

Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes. Ackers-Johnson M, Foo RS. Methods Mol Biol. 2019;1940:193-204.

Here, we outline a convenient, alternative approach to isolation and culture of viable myocytes and non-myocytes from the adult mouse heart, developed in our lab. The technique requires only common surgical and laboratory equipment, and involves direct needle perfusion of the left ventricle ex vivo. Viable myocyte yields and functionality are comparable to those described in established Langendorff-based protocols.

Since publication, this procedure has been reproduced and established in other labs, worldwide. We continue to collaborate and advise on implementation and troubleshooting, please get in touch!

Citations

(Selected citations from 57 to-date)

Glycogen Synthase Kinase-3α Promotes Fatty Acid Uptake and Lipotoxic Cardiomyopathy. Nakamura M, Liu T, Husain S, Zhai P, Warren JS, Hsu CP, Matsuda T, Phiel CJ, Cox JE, Tian B, Li H, Sadoshima J. Cell Metab. 2019;29:1119-1134.e12

Robust CTCF-Based Chromatin Architecture Underpins Epigenetic Changes in the Heart Failure Stress-Gene Response. Lee DP, Tan WLW, Anene-Nzelu CG, Lee CJM, Li PY, Luu TDA, Chan CX, Tiang Z, Ng SL, Huang X, Efthymios M, Autio MI, Jiang J, Fullwood MJ, Prabhakar S, Lieberman Aiden E, Foo RS. Circulation. 2019;139:1937-1956

The N6-Methyladenosine mRNA Methylase METTL3 Controls Cardiac Homeostasis and Hypertrophy. Dorn LE, Lasman L, Chen J, Xu X, Hund TJ, Medvedovic M, Hanna JH, van Berlo JH, Accornero F. Circulation. 2019;139:533-545

Megakaryocytic Leukemia 1 Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Yu L, Yang G, Zhang X, Wang P, Weng X, Yang Y, Li Z, Fang M, Xu Y, Sun A, Ge J. Circulation. 2018;138:2820-2836

Targeting Chondroitin Sulfate Glycosaminoglycans to Treat Cardiac Fibrosis in Pathological Remodeling. Zhao RR, Ackers-Johnson M, Stenzig J, Chen C, Ding T, Zhou Y, Wang P, Ng SL, Li PY, Teo G, Rudd PM, Fawcett JW, Foo RSY. Circulation. 2018;137:2497-2513

Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion. Edwards KS, Ashraf S, Lomax TM, Wiseman JM, Hall ME, Gava FN, Hall JE, Hosler JP, Harmancey R. Basic Res Cardiol. 2018;113:47

Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo. See K, Tan WLW, Lim EH, Tiang Z, Lee LT, Li PYQ, Luu TDA, Ackers-Johnson M, Foo RS. Nat Commun. 2017;8:225

Paracrine effect of regulatory T cells promotes cardiomyocyte proliferation during pregnancy and after myocardial infarction. Zacchigna S, Martinelli V, Moimas S, Colliva A, Anzini M, Nordio A, Costa A, Rehman M, Vodret S, Pierro C, Colussi G, Zentilin L, Gutierrez MI, Dirkx E, Long C, Sinagra G, Klatzmann D, Giacca M. Nat Commun. 2018;9:2432

A landscape of circular RNA expression in the human heart. Tan WL, Lim BT, Anene-Nzelu CG, Ackers-Johnson M, Dashi A, See K, Tiang Z, Lee DP, Chua WW, Luu TD, Li PY, Richards AM, Foo RS. Cardiovasc Res. 2017;113:298-309

With or Without Langendorff: A New Method for Adult Myocyte Isolation to Be Tested With Time. Chen X, O’Connell TD, Xiang YK. Circ Res. 2016;119:888-90